Explore
Validate
Learn
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- CSI 001-76-02 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Perlecan Monoclonal Antibody (A76)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- CSI 001-76-02 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). CSI 001-76-02 cross-reacts with human thrombospondin. Other species have not been tested.
- Antibody clone number
- A76
- Concentration
- 1.01 mg/mL
Submitted references Similarity of recombinant human perlecan domain 1 by alternative expression systems bioactive heterogenous recombinant human perlecan D1.
Ellis AL, Pan W, Yang G, Jones K, Chuang C, Whitelock JM, DeCarlo AA
BMC biotechnology 2010 Sep 9;10:66
BMC biotechnology 2010 Sep 9;10:66
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 ELISA identifies only Pln.D1 but not Pln.D3-5 in enriched rhPln.198 . The enriched high M r rhPln.198 synthesized by HEK 293 cells was used to coat microtiter wells that were subsequently blocked then incubated with 1.7 mug/ml of different primary antibodies as depicted in the legend. ELISA was completed as described in Methods. Background signal for each antibody binding to uncoated wells was subtracted to produce the net signal. Inset: ELISA data showing negligible mAb A76 reactivity against rhPln.247 and strong reactivity with full-length endothelial perlecan (purified as previously described [ 53 ]), while mAb A71 reacted strongly to both the native perlecan and the truncated recombinant. Net absorbance minus buffer-coated wells represented.
